Abstract: Microalgae carbon sequestration plays a crucial role in addressing greenhouse effect, energy crisis, and promoting low-carbon economic development. However, low carbon sequestration efficiency is the bottleneck problem limiting the development of this technology. Therefore, this article proposes the use of organic amine supported hollow fiber membranes （hydrophobic polypropylene （PP） membranes） to improve microalgae carbon sequestration performance. Firstly, hydrophobic modification is performed on the hollow fiber membrane, followed by physical loading of 2-amino-2-methyl-1-propanol （AMP） onto the hollow fiber membrane; Then, immerse it in a microalgae culture system as a medium for CO₂ gas to enter the culture medium. During the process, AMP loaded PP membrane can enhance the mass transfer of CO₂ to microalgae culture medium, thereby improving the efficiency of CO₂ removal; AMP loaded PP membrane can serve to separate the chemical reaction field between CO₂ and amines, as well as the biological reaction field for microalgae to absorb CO₂, avoiding complex mixing reactions; The AMP on the PP membrane and the amino formate formed by the reaction between AMP and CO₂ do not dissolve in the culture medium and do not directly contact microalgae cells, greatly reducing damage to microalgae cells and not affecting cell photosynthesis, thereby improving efficiency; The AMP loaded PP membrane and microalgae system exhibit a separation state, which can be separated by simple physical methods. The experimental results showed that the 123.16 mg/L AMP-PP membrane reinforcement technology can significantly increase biomass concentration （0.88 g/L） and CO₂ fixation efficiency （183.23 mg/（L·d））. Compared with the Blank group, Blank PP group, and 123.16 mg/L AMP group, the biomass concentration increased by 28.05%, 18.04%, and 183.57%, respectively, and the CO₂ fixation efficiency increased by 58.23%, 44.16%, and 168.42%, respectively. At the same time, this enhanced technology eliminates oxidative stress （decreased SOD activity） and cell damage caused by direct contact with AMP through physical isolation （fractal dimension increased by only 5.9%）. The amino formate generated after CO₂ adsorption on the surface of the aminated membrane increases the concentration of dissolved inorganic carbon （DIC） in the culture medium through hydrolysis reaction, and the CO₂ removal efficiency reaches 83.5%, significantly better than the dissolved AMP system, highlighting the effectiveness of this membrane mediated method in microalgae CO₂ fixation.